Journal: Molecular Medicine Reports
Article Title: Optimal combination of cationic lipid and phospholipid in cationic liposomes for gene knockdown in breast cancer cells and mouse lung using siRNA lipoplexes
doi: 10.3892/mmr.2022.12769
Figure Lengend Snippet: Effect of phospholipid in cationic liposomes on biodistribution of siRNA in mice at 1 h after systemic injection of siRNA lipoplexes. siRNA lipoplexes with 20 µg Cy5-siRNA were administered intravenously to mice. Cy5 fluorescence imaging of tissue was performed 1 h post-injection. Fluorescence intensity is illustrated using a color-coded scale (red, maximum; purple, minimum). Scale bar, 1 cm. si, small interfering; Cy5, cyanine 5; DDAB, dimethyldioctadecylammonium bromide; DOTAP, 1,2-dioleoyl-3-trimethylammonium-propane methyl sulfate salt; TC-1-12, 11-[(1,3-bis(dodecanoyloxy)-2-((dodecanoyloxy)methyl)propan-2-yl)amino]-N,N,N-trimethyl-11-oxoundecan-1-aminium bromide; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; POPE, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine.
Article Snippet: The localization of Cy5-siRNA was examined using a fluorescent microscope (Eclipse TS100-F; Nikon Corporation) with optical filter Cy5 HQ (excitation, 620/60 nm; dichroic mirror, 660 nm; emission, 700/75 nm; Nikon Corporation).
Techniques: Injection, Fluorescence, Imaging